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Archive > Volume 23 Issue 4 > 2016,23(4):476-480. DOI:10.3872/j.issn.1007-385X.2016.04.003 Prev Next
Prompt Report
Function analysis of MAGE-C2 antigen-specific CD8+ T cells in peripheral blood of patients with esophageal cancer and its clinical significance
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Abstract:
Objective:To evaluate the function of melanoma-associated antigen (MAGE)-C2 antigen-specific CD8+T cells in peripheral blood of the patients with esophageal cancer, and analyze its relevance to clinical pathological parameters. Methods:Peripheral blood mononuclear cells (PBMCs) were obtained from 130 patients with esophageal cancer who were collected by Department of thoracic surgery, the First Hospital affiliated to Zhengzhou University during November 2014 and November 2015 with density gradient centrifugation. The patients with co-positive HLA-A2*0201 and MAGE-C2 were selected with flow cytometry and RT-PCR assays. After PBMCs of the patients were cultured for 7 and 14 days and treated with MAGE-C2(336-344) antigen peptide (1 μg/ml), IL-12 (100 μg/ml) and CD3/CD28 Dynabeads (2 μl) to induce amplification of antigen-specific CD8+ T cells, as well as CD107a on surface of CD8+ T cells and IFN-γ in the cells were detected with flow cytometry assay, and their relationships with clinical parameters were analyzed. Results: Fifty two patients with co-positive HLA-A2*0201 and MAGE-C2 were selected out. After their PBMCs were treated with MAGE-C2(336-344), percentage of CD107a+ IFN-γ+ / CD107a+ IFN-γ-/ CD107a-IFN-γ+ in CD8+ T cells of the treatment group was obviously increased (P<0.05). Expression of CD107a in early stage and highly differentiated esophageal cancer patients without lymphatic metastasis was obviously higher than that in advanced and poorly differentiated esophageal cancer patients with lymphatic metastasis (P<005). Expression of IFN-γ in patients with highly differentiated esophageal cancer was higher than that in patients with poorly differentiated esophageal cancer (P<0.05). Conclusion: Amplification of MAGE-C2 antigen-specific CD8+ T cells was successfully introduced, and associations of its function with esophageal cancer staging and lymphatic metastasis were found. It could become an ideal target for immunotherapy of the cancer.
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Project supported by the National Natural Science Foundation of China (No. 81171986; 81271815), the National Natural Science Foundation of China and Sino-US Cooperation in Biomedical Research Projects (No. 812111102), the Research Grant from the Ministry of Public Health (No. 20110110001), and the Basic and Advanced Technology Research Foundation from Science and Technology Department of Henan Province (No. 112300410153; 122300410155)
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