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Archive > Volume 23 Issue 4 > 2016,23(4):481-485. DOI:10.3872/j.issn.1007-385X.2016.04.004 Prev Next
Basic Research
gene inhibit migration and invasion abilities of non-small cell lung cancer A549 cell through NF-κB pathway
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Abstract:
Objective:To study the effect of bridging intergrator-1 (Bin1) gene over-expression on migration and invasion abilities of non-small cell lung cancer A549 line cell, and initially discuss mechanism of its action. Methods: Eukaryotic expression plasmid CMV-MCS-GFP-SV40-Neomycin-Bin1 containing full length Bin1 gene sequence was transfected into the A549 cell using cationic liposomes and gene transfection technology (as Bin1 transfection group), as well as blanck control and empty plasmid transfection groups were set up. Expressions of Bin1mRNA and protein in various treatment groups were detected with PT-PCR and Western blotting assays. Effects of Bin1over-expression on migration and invasion abilities of the A549 cell were examed by scarification and Transwell invasion tests respectively. Western blotting assay was used to detect effects of Bin1over-expression on NF-κB phosphorylation level and expressions of migration-related proteins, E-cadherin, N- cadherin and MMP-9, in the A549 cell.Results: Comparing with blanck control and empty plasmid transfection groups, expressions of Bin1 mRNA and protein in the A549 cell of Bin1 transfection group increased significantly (all P<0.05). Cell abilities of migration and invasion in Bin1transfection group were significantly lower than those in empty plasmid transfection and blanck control groups (number of penetrating cells: \[50.50±3.15\] vs \[124.00±425\], \[130.00±4.37\], all P<0.05). In the A549 cell of Bin1transfection group, expressions of NF-κB and E-cadherin obviously increased (all P<0.05), and expressions of p-NF-κB, N- cadherin and MMP-9 significantly decreased (all P<0.05), compared to empty plasmid transfection and blanck control groups. Conclusion: Over-expression of Bin1 could inhibit abilities of migration and invasion of the A549 cell, and its mechanism might be associated with inactivation of NF-κB pathway and expression changes of cell migration and invasion-related proteins.
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Project Supported:
Project supported by the Young Scientists Foudation of the National Natural Science Foudation of China（No.81201607），the Outstanding Youth Foudation of Hebei Province（No.H2014206320），and the Training Foundation of High Level Talents of Hebei Province（No. A201401040）
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