Mobile website
Archive > Volume 23 Issue 4 > 2016,23(4):492-497. DOI:10.3872/j.issn.1007-385X.2016.04.006 Prev Next
Basic Research
CD44 postive cell in gastric cancer cells possesses the characteristics of cancer stem cell
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective:To confirm cancer stem cell characteristics of CD44+ gastric cancer cell in orthotopic carcinoma xenograft model. Methods: MKN-45 gastric cancer line cells were separated into two cell groups, CD44+ and CD44-, with flow cytometry assay, as well as non-separated MKN-45 gastric cancer cell as control. Proliferation in vitro of CD44+ gastric cancer cell was detected by MTT and colony formation assays and its invasion ability in vitro tested by Transwell assay. The above gastric cancer cells were injected into nude mice by orthotopic tansplantation method. The transplanted nude mice were killed and detected for their rates of tumor formation after breeding for 8 weeks under the same conditions. Their livers were taken out and counted the number of hepatic metastasis. Shapes of gastric tumor and metastatic tumor in liver were observed with H-E staining. Numbers of the CD44+ cell in both the tumors were counted by immunofluorescence assay. Results: Compared with CD44- gastric cancer cell, proliferation and invasion abilities of CD44+ gastric cancer cell obviously enhanced ( all P<0.01), formation of tumor and metastasis more frequently occurred (P<005), but compared with non-separated MKN-45 gastric cancer cell there not were any statistic differences (P>0.05). Whether in primary tumor or in metastatic one, CD44+ gastric cancer cells could all produce CD44- gastric cancer cells, but the latter could not produce the CD44+ cells. Conclusion: Compared with CD44- gastric cancer cells, CD44+ MKN-45 gastric cancer cells could have more stronger proliferation and invasion abilities, and be more likely to become tumor and metastasis, which meet partial characteristics of cancer stem cell. It might be valuable to further investige.
Keywords:
Project Supported:
Project support by Laboratory Animal Program of Science and Tachnology Commission of Shanghai （No. 13140901803），Chinese Troditional Medicine-induced Scientific Tachnology Program of Science and Tachnology Commission of Shanghai （No. 13401907500）, and the National Natural Science Foundation of China（No. 81503391）
Clc Number:
