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Archive > Volume 23 Issue 4 > 2016,23(4):506-509. DOI:10.3872/j.issn.1007-385X.2016.04.008 Prev Next
Basic Research
Effect of down-regulating microRNA-214 expression on migration and invasion of ovarian carcinoma SKOV-3 cells
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Abstract:
Objective:To observe effect of down-regulating microRNA-214 (miR-214) expression on migration and invasion of ovarian carcinoma SKOV-3 cells and explore its possible mechanism. Methods: miR-214 inhibitors were transfected into human ovarian carcinoma SKOV-3 cells by liposome. Expressions of miR-214, nuclear factor-κB (NF-κB) and urokinase-type plasminogen activator (uridylyl phosphate adenosine \[uPA\]) gene mRNA, as well as NF-κB and uPA proteins in the cells were respectively detected by Real-time PCR and Western blotting assays. Scarification test and transwell assay were used to detect abilities of migration and invasion of the cells respectively. Results: After transfection of miR-214 inhibitor, expression of miR-214 in human carcinoma SKOV-3 cells decreased, and migration and invasion abilities of the cells reduced. At the same time, expressions of NF-κB and uPA gene mRNA and proteins also depressed. Conclusion: Down-regulation of miR-214 expression in human ovarian carcinoma SKOV-3 cells could inhibit migration and invasion abilities of the cells, and down-regulation of NF-κB and uPA gene expressions might be its possible mechanism.
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Project Supported:
Project supported by Foundation of Innovative Research Team in the Logistics College of Armed Police Force (No.whtd2013-2）
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