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Archive > Volume 23 Issue 4 > 2016,23(4):525-530. DOI:10.3872/j.issn.1007-385X.2016.04.012 Prev Next
Clinical Research
Expressions of OCT4 and EMT-associated factor in invasion breast cancer and their clinical significances
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Abstract:
Objective:To detect expressions of mRNAs and proteins for EMT-assosiated factors, E-cadherin and vimentin, and stem cell factor OCT4 in invasive breast cancer and correlation of OCT4 with E-cadherin and vimentin. Method: Fourty samples of invasive breast cancer tissues were collected. Expressions of mRNAs and proteins for EMT-assosiated factors, E-cadherin and vimentin, and stem cell factor OCT4 were respectively detected by Real-time quantitative PCR and immune histochemistry assay. Relationship between expressions of the mRNA and the proteins, and survival rates and clinical pathological features of the patients with invasive breast cancer, and correlation of OCT4 with E-cadherin and vimentin, were analyzed. Results:In the invasive breast cancer tissues, expression rates of OCT4, E-cadherin and vimentin were 30% (12/40), 55% (22/40), and 65% (26/40) respectively. The expression of OCT4 was correlated with age, histological grade, lymph node metastasis and Her-2 expression of the patients with invasive breast cancer. Expression of E-cadherin was related with lymph node metastasis and expression of vimentin was related with histological grade and lymph node metastasis. Expressions of mRNA and protein for OCT4 and E-cadherin were negatively correlated. Expressions of mRNA and protein for OCT4 and vimentin were positively correlated. Also expressions of mRNA and protein for E-cadherin and vimentin were negatively correlated. Survival rates of the patients with positive expression of OCT4 were significantly lower than those of the patients with negative expression of OCT4 (P<0.05). However there were no significant differences between expressions of E-cadherin and vimentin, and survival rates of the patients (P>0.05). Conclusion: Stem cells factor OCT4 might promote metastasis of the invasive breast cancer cells via EMT, and might be a factor affecting prognosis of the invasive breast cancer.
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Project Supported:
Project support by Young Scientist Program of the National Natural Scientific Foundation of China （No. 81502032)
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