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Archive > Volume 23 Issue 5 > 2016,23(5):703-707. DOI:10.3872/j.issn.1007-385X.2016.05.020 Prev Next
Technigue and Method
Establishment of an endogastric xenograft tumor modal in nude mice and its detection by in vivo fluorescence imaging system
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Abstract:
Objective:To establish an endogastric xenograft tumor model in nude mice which can be dynamically monitored by in vivo fluorescence imaging system. Methods: The SGC-7901fLuc+ cell line stably expressing luciferase was injected subcutaneously into nude mice to develop transplanted tumor. Subcutaneously transplanted tumor (with diameter of 0.5 cm) was collected and orthotopically overlapping sutured into lesser gastric curvature of nude mice. Tumor growth in rats was monitored using in vivo fluorescence imaging system every 4 days. At 3 weeks post-tumor implantation, the fluorescence-positive mice were sacrificed and H-E staining was performed to observe the morphology of endogastric xenograft tumor. Results:Using in vivo fluorescence imaging system, gradually enhanced fluorescence signal could be detected on the gastric area of tumor bearing nude mice. Tumor block grew locally around the lesser gastric curvature, with typical gastric carcinoma morphology as assessed by H-E staining. The diameter of the tumor block was 0.5-1 cm. The boundary of tumor and adjacent tissue was clear. No adhesion, no metastasis was found in the abdominal cavity, and no formation of ascites was found. The successful rate of endogastric xenograft tumor model of human gastric carcinoma in nude mice was 95% (19/20). Conclusion: The endogastric xenograft tumor model in nude mice was successfully established. This model provided an ideal experimental tool for studying the mechanism of human gastric carcinoma and the development of anticancer drugs.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No. 81372459）
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