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Archive > Volume 23 Issue 6 > 2016,23(6):773-778. DOI:10.3872/j.issn.1007-385X.2016.06.006 Prev Next
Basic Research
miR-33a inhibits invasion and migration of colon cancer cells through down-regulation of lumican
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Abstract:
Objective:To investigate expression situations of miR-33a and lumican in colon cancer at various stages and to explore effect of miR-33a on invasion and migration of colon cancer cells. Methods: Expression situations of miR-33a and lumican in colon cancer at various sages were detected by Western blotting and qPCR. To up-regulate expression of miR-33a, miR-33a mimic was transfected into colon cancer SW480 line cells. Expressions of miR-33a and lumican mRNAs after the transfection of miR-33a mimic were detected by qPCR. Transwell and scratch assays were used to examine effect of miR-33a on invasion and migration abilities of human colon cancer SW480 line cells respectively. Subcutaneous tumor formation assay in nude mice was used to detect growth of tumor tissues in the colon cancer and survival of the nudemice.Results: With increased tumor stages of the colon cancer, expression of miR-33a obviously decreased, and expression of lumican obviously increased. As increasing pathological stage and grade, expression of miR-33a gradually decreased. Expression of miR-33a evidently decreased in the colon cancer tissues with lymph node metastasis. Using of miR-33a mimic clearly up-regulated expression of miR-33a, which significantly decreased expression of lumican protein. Up-regulation of miR-33a inhibited invasion and migration abilities of the colon cancer cells. Cancer growth extent of the tumor bearing mice in the miR-33a mimic group obviously decreased, and their survival period evidently prolonged. Conclusion: miR-33a could be associated with staging and grading of the colon cancer as well as lymph node metastasis. miR-33a could inhibit invasion and migration of the colon cancer through inhibition of lumican.
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Project Supported:
Project supported by Program of Health Bureau of Henan Province (No. 1203068)
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