Mobile website
Archive > Volume 23 Issue 6 > 2016,23(6):779-782. DOI:10.3872/j.issn.1007-385X.2016.06.007 Prev Next
Basic Research
Effect of sporamin on expression of Notch4 protein in human pancreatic cancer cells and proliferation, migration, invasion of the tumor cells
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To investigate effect of trypsin inhibitor, sporamin, on expression of Notch4 protein in human pancreatic cancer (PC) cells and proliferation, migration, invasion of the tumor cells. Methods: Effect of the sporamin on proliferation of the PANC-1 and MiaPaCa-2 PC line cells was detected by MTT assay; proliferation and invasion abilities in vitro of the pancreatic cancer cells detected by Transwell assay and effects of various concentrations of the sporamin (0，25，50，75 and 100 μg/ml) on expression level of the Notch4 protein in the human pancreatic cancer cells detected by Western blotting assay. Results: Sporamin significantly inhibited prolilferation, migration and invasion abilities of the PANC-1and MiaPaCa-2 PC line cells, as a patern of concentration-dependence (P<0.05), but not a patern of time-dependence (P>0.05). With increase of the sporamin concentration, expression level of the Notch4 protein also decreased gradually. Conclusion:Trypsin inhibitor sporamin could inhibit proliferation, migration and invasion abilities of the PC cells with a concentration-dependence, and simualtaneously inhibit expression of the Notch4 protein in the PC cells.
Keywords:
Project Supported:
Project supported by the Natural Science Foundation of Zhejiang Province (No. LY16H160033)， and the Program of Application Research on Public Welfare Technology of Zhejiang Province （No.2016C33189）
Clc Number:
