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Inhibitory effect of FOXQ1 gene silencing on migration and invasion abilities of hepatocellular carcinoma cell line SMMC-7721
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Abstract:
Objective:To explore the effect and mechanism of Forkhead box Q1 (FOXQ1) gene silencing on migration and invasion abilities of hepatocellular carcinoma cell line SMMC-7721. Methods: FOXQ1-shRNA and NC-shRNA recombinant lentiviral vectors were constructed and transduced into SMMC-7721 cells. There were interfered group, negative group and blank group. Transwell chamber assay was used to detect the abilities of cell migration and invasion. qRT-PCR and Western blotting were used to examine the mRNA and protein expressions of FOXQ1, MMP-2 and MMP-9 in SMMC-7721 cells.Results: FOXQ1 expression level was positively correlated to the migration and invasion abilities of SMMC-7721 cells; Compared with the negative group and blank group, the expression level of FOXQ1 was decreased in interfered group (\[0.34±0.03\] vs \[0.89±0.07\] and \[0.84±0.05\], P<0.05); After FOXQ1gene silencing, the cell migration and invasion abilities of SMMC-7721 cells were significantly decreased (\[9.67±1.15\] vs \[25.67±2.08\] and \[2733±252\]，P<0.05\], in the meanwhile, the expressions of MMP-2 and MMP-9 were down-regulated significantly \[MMP-2: \[0.35±0.04\] vs \[0.61±0.05\] and \[0.65±0.08\]；MMP-9: \[0.40±0.05\] vs \[0.73±0.07\] and \[0.77±006\], all P<0.05). Conclusion: FOXQ1 gene silencing could suppress migration and invasion abilities of hepatocellular carcinoma cell line SMMC-7721. Its mechanism might be related to the down-regulation of MMP-2 and MMP-9.
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Project Supported:
Project supported by the Scientific Research Foundation of the Education Bureau of Sichuan Province（No. 12ZB218）
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