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Clinical Research
Expression of PD-1 in CIK cells derived from umbilical cord blood or peripheral blood from breast cancer patients and the cytotoxicity of CIKs on MCF-7 cells
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Abstract:
Objective:To detect the expressions of programmed cell death-1 (PD-1) on cytokine-induced killer (CIK) cells derived from umbilical cord blood or peripheral blood from breast cancer patients, as well as to investigate the cytotoxicity of CIK cells on MCF-7 cells. Methods: Umbilical cord blood from healthy pregnant women (n=5) and venous blood from breast cancer patients (n=5) were collected during June 2015 to December 2015 at the 105th Hospital of PLA. The PBMC was isolated, and CIK cells were differentiated and amplified in vitro. The PD-1 expressions on CIK cells derived from two origins at different time points were detected by FCM; CIK cells at 7th, 14th , 21st and 28th days were used for the co-culture with MCF-7 cells, and the cytotoxicity of CIK cells on MCF-7 cells was determined by CCK-8 assay; CIK cell apoptosis after co-culture was observed by AOEB, and the apoptosis rate of CIK cells was determined by FCM. Results：Along with the extending of incubation time, the expressions of PD-1 on CIK cells of both groups increased gradually; PD-1 expression in CIK cells devived from umbilical cord blood at 14th day was lower than that devived from peripheral blood of breast cancer patients (\[38.42±4.76\]% vs \[50.54±3.50\]%,P>0.05), however, the expressions increased in both groups at 21st day and the difference between two groups was not statistically significant (P>005). Cytotoxicity rates of the CIK cells on the MCF-7 cell in the two groups at 7th ，14th ，21st and 28th days after co-culture were (18.54±3.54)% and (21.74±4.27）%，（71.86±16.86）% and（58.78±24.25）%，（44.32±2687）% and （43.96±26.04）% as well as （43.24±24.27）% and （40.28±23.69）% respectively, and among them the cytotoxicity of the CIK cells from umbilical cord blood at 14th day of the culturing was the highest (P<0.05). According to the analysis, there was a positive correlation between PD-1 expression and CIK cell apoptosis(r=0.971,r=0.900, all P<0.01), and a negative correlation between PD-1 expression and CIK cytotoxicity rate (r=-0.865，r=-0.885, all P<0.01). Conclusion：The activated CIK cells had high PD-1 expression，and CIK cells from patients with breast cancer had higher PD-1 expression than CIK cells from umbilical cord blood. The apoptosis rates of CIK cells at 14th day of culture were in both groups lower, and possessed higher cytotoxicities.
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Project Supported:
Project supported by the Science and Technology Innovation Foundation of Nanjing Military Region（No.15MS048）
Clc Number:
