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Archive > Volume 24 Issue 4 > 2017,24(4):423-428. DOI:10.3872/j.issn.1007-385X.2017.04.015 Prev Next
Clinical Research
Expression of ubiquitin specific peptidose 53 in colorectal cancer tissues and its inhibitory effect on HCT116 cells
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Abstract:
Objective:To investigate the expression of ubiquitin specific peptidose 53（USP53） in colorectal cancer tissues and its over-expression on human colorectal cancer(CRC) HCT116 cells. Methods: Real-time PCR and IHC were used to examine the expression level of USP53 in colorectal cancerous tissues and the para-cancerous tissues; TCGA database, provided by UCSC CANCER BROWSER, was used to validate the relationship between USP53 mRNA expression and clinical characteristics as well as prognosis; After over-expressing USP53in HCT116, the changes in cell proliferation were measured by CCK-8 assay and colony formation assay. Results: IHC showed that the expression level of USP53 was higher in the normal para-cancerous tissues than that in cancerous tissues (91.67%\[44/48\] vs 18.75% \[9/48\], P<001); the same was also found in the mRNA level (\[0.46±0.27\] vs \[0.85±0.32\], F=0.925, P<0.05). The mRNA level of USP53in CRC was negatively correlated with the prognosis (P<0.05); Over-expression of USP53 in HCT116 cell significantly decreased the colony formation (\[123±27.22\] vs \[338±55.24\]，P<0.01); CCK-8 assay also showed that over-expression of USP53 also significantly inhibited the proliferation of HCT116 cells (\[0.14±0.01\] vs \[0.18±0.04\]，P<0.05, \[0.23±0.01\] vs \[0.32±0.01\]，P<0.01，\[0.45±0.03\] vs \[0.80±0.05\]，P<001，\[0.83±0.03\] vs \[1.18±0.10\]，P<0.01). Conclusion: USP53 was downregulated in CRC, which is correlated with the poor prognosis; and up-regulation of USP53 could inhibit the proliferation of HCT116 cells in vitro, indicating USP53 could be used as a diagnostic and therapeutic target for colorectal cancer.
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Project supported by the National Natural Science Foundation of China (No. 81372636 and No.81302089), the National High Technology Research and Development Program of China (863 Program) (No. SQ2014SFOZD00314), and the Shanghai Excellent Young Teachers Program (No. ZZjdyx13074)
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