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Archive > Volume 24 Issue 5 > 2017,24(5):521-526. DOI:10.3872/j.issn.1007-385X.2017.05.011 Prev Next
Basic Research
Effect of Klotho gene on malignant biological behavior of cervical squamous cells and its mechanism
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Abstract:
Objective:To investigate the effect and mechanism of Klotho gene on the biological characteristics of cervical squamous cell carcinoma SiHa cells. Methods: The expression of Klotho in the cervical squamous cell carcinoma cell SiHa, HeLa and C33A lines and the human immortalized epidermal cell HaCaT line was detected by Real-time PCR and Western blotting. Vectors over-expressing Klotho were constructed and used to transfect SiHa cells, and then the cell proliferation, apoptosis and migration were detected with CCK-8 assay, Flow cytometry and Transwell assay, respectively. The expressions of Rho A and ROCK 1 at protein level were measured with Western blotting assay. Results: Expressions of Klotho in cervical cancer cell SiHa, Hela and C33A lines were lower than that in the HeCaT line (P<0.05). After over-expression of Klotho in SiHa cells, (1) cell proliferation was significantly decreased, compared with control group (\[67.37±5.04\]% vs \[100.34±7.62\]%, P<0.05); (2) the percentage of cells at G0/G1 phrase was increased(\[61.37±3.28\]% vs \[82.56±3.89\]%, P<0.05), while the percentage at S phrase was decreased (\[9.12±248\]% vs \[28.97±2.08\]%, P<0.01); (3) cell apoptotic rate at both early and late stage were all decreased (all P<0.05); (4) cell migration was suppressed (P<0.01); (5) the expressions of Rho A and ROCK 1 at protein level were significantly decreased (all P<0.01). Conclusion: Klotho suppressed SiHa cell proliferation and migration, and promoted cell apoptosis; the mechanism may be related to the suppression of Rho A/ROCK 1 signaling pathway activation, suggesting that Klotho may be used as a potential new target for diagnosis and targeted therapy of cervical squamous cell carcinoma.
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