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Archive > Volume 24 Issue 10 > 2017,24(10):1076-1080. DOI:10.3872/j.issn.1007-385X.2017.10.006 Prev Next
Basic Research
Effect of combined treatment of cortex periplocae and TRAIL on gastric cancer SGC-7901 and MGC-803 cells and its mechanism
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Abstract:
Objective:To investigate the effect of combined treatment of cortex periplocin (CPP) and tumor necro-sis factor related apoptosis inducing ligand (TRAIL) on gastric cancer cells and to explore the mechanism. Meth-ods: After routine culture, the gastric cancer cell lines (SGC-7901 and MGC-803) were treated with CPP (at concen-trations of 50，100，200 ng/ml) or TRAIL (1 μg/ml) or in combination of these two. The cell proliferation was detect-ed by MTS, the apoptosis was detected by Flow cytometry, and the expression levels of pro-BID，Mcl-1，cleaved caspase-3，DR4 and DR5 were detected by Western blotting. Results: Compared with the control group and each CPP single treatment group, MTS assay demonstrated that CPP (50，100，200 ng/ml) in combination with TRAIL (1 μg/ml) significantly inhibited the proliferation of gastric cancer SGC-7901 and MGC-803 cell lines (all P<0.05 or P<0.01). Flow cytometry demonstrated that the apoptosis rate of gastric cancer cells in combined treatment group was significantly higher than that of control group or each CPP single treatment group (P<0.05 or P<0.01). Western blotting demonstrated that combined treatment for 24 h significantly decreased the expression of pro-BID and Mcl-1 (P<0.05), but increased the expression levels of cleaved caspase-3，DR4 and DR5(P<0.05). Conclusion: CPP in combination with TRAIL could significantly induce the apoptosis of gastric cancer SGC-7901 and MGC-803 cell lines and increase the susceptibility of cancer cells to TRAIL.
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Project Supported:
Project supported by the National Natural Science Foundation of China（No.81673642)
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