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Archive > Volume 24 Issue 12 > 2017,24(12):1403-1408. DOI:10.3872/j.issn.1007-385X.2017.12.011 Prev Next
Clinical Research
miR-22 and miR-126 expression in serum of patients with non-small cell lung cancer and its clinical significance
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Abstract:
Objective: To investigate the expression of microRNA-22 (miR-22) and miR-126 in blood serum of patients with non-small cell lung cancer (NSCLC) and explore their role in the occurrence and metastasis of NSCLC.Methods: A total of 127 NSCLC patients who were diagnosed at the Sixth People's Hospital of Yancheng City during May 2013 till May 2015 were selected in this study, meanwhile another 112 healthy individuals were selected as the control subjects. The qPCR was performed to detect the serum miR-22 and miR-126 levels. Logistic regression analysis was conducted to analyze the independent factors influencing NSCLC metastasis, and receiver operating characteristic (ROC) curve was drawn to analyze the sensitivity and specificity of serum miR-22 and miR-126 levels in predicting NSCLC developments and metastasis. Results: The serum miR-22 level was significantly higher in the case group than that in the control group, while the serum miR-126 level was lower in the case group as compared with that in the control group (all P<0.05). Compared with squamous cell carcinoma patients, serum miR-22 level significantly increased (P<0.05), while serum miR-126 level decreased in patients with adenocarcinoma (P<0.01). Patients at III+IV stage showed increased serum miR-22 level and decreased serum miR-126 level as compared to patients at I + II stage (all P<0.01). In comparison to those without metastasis, patients with metastasis showed elevated serum miR-22 and decreased miR-126 level (all P<0.01). Compared with patients with familial inheritance,those without familial inheritance had increased serum miR-22 level but decreased serum miR-126 level (all P<0.01). The specificity and sensitivity of serum miR-22 and miR-126 levels in predicting NSCLC occurrence were 99.11% and 84.30%, 82.68% and 96.40%, respectively. The specificity and sensitivity of serum miR-22 and miR-126 levels in predicting NSCLC metastasis were 59.74% and 96.00%, 84.00% and 62.30%, respectively. Conclusion:miR-22 was high expressed while miR-126 was low expressed in the serum of NSCLC patients; they may be used as the predicative biomarkers for NSCLC development and metastasis.
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Project Supported:
Project supported by the National Natrural Science Foundation of China（No. 81460247）, the First-class Discipline Construction of Clinical Medicine of Ningxia Medical University（No. YXW2017014）, the Plan of Postgraduate Education Innovation, Discipline Construction Project of Ningxia (2017) (No. YXW2017014), the 2015 Postgraduate Education Innovation Program of Ningxia Medical University（No. NXYC201511）,and the 2016 Medical Science and Technology Development Program of Yancheng City（No. 81460247）
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