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Archive > Volume 24 Issue 12 > 2017,24(12):1414-1418. DOI:10.3872/j.issn.1007-385X.2017.12.013 Prev Next
Clinical Research
miR-205-5p expression in peripheral blood karyocytes from non- small cell lung cancer patients and its clinical value
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Abstract:
Objective: To investigate whether miR- 205- 5p expression in peripheral blood karyocytes from nonsmall cell lung cancer (NSCLC) patients could be used as a reasonable molecule biomarker. Methods: Peripheral blood from 60 patients, who were primarily diagnosed with NSCLC and treated at the Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital) from June, 2011 to June, 2012, was collected; among them,there were 30 cases with distant metastasis while the other 30 without. In addition, 30 healthy subjects were enrolled as controls. The miR- 205- 5p expressions in collected peripheral blood karyocytes, human bronchial epithelial BEAS-2B cells, Gejiu squamous cell lung carcinoma YTMLC-90 cells, and Xuanwei lung adenocarcinoma XWLC-05 cells were detected by qPCR. We also analyzed the correlation between miR-205- 5p expression in peripheral blood karyocytes and NSCLC serum markers levels, TNM stage, lymph node metastasis, as well as distant metastasis etc. Results: The expressions of miR-205-5p in YTMLC-90 (17.507 2±1.906 3) and XMLC-05 cells (5.725 2±1.012 0) were significantly higher than that in BEAS-2B cells (0.820 8±0.255 3) (P<0.05). The miR-205-5p expres-sion level in peripheral blood karyocytes from healthy controls was significantly higher than that from NSCLC patients (P<0.01). The positive rate of miR-205-5p in peripheral blood karyocytes from NSCLC patients was not correlated with patients’sex, age, lymph node metastasis, pathologic subtypes and tumor serum markers (CEA，CA125,CA242) (all P>0.05), but significantly correlated with distant metastasis, TNM stage and tumor serum marker CA153 (P<0.01). Conclusion: Abnormal up-regulation of miR-205-5p may play an important role in the progression of NSCLC and can be used as a reasonable biomarker for the metastasis in NSCLC patients.
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Project Supported:
Project supported by the National Natural Science Foundation of China (No. 81560380, No.81460358, No.81060177), the Yunnan Provincial Technology Project of Health (No. 2014NS023, No. 2014NS024), and the Zhan Qimin Academician Workstation of Yunnan Province (No. 2015IC002)
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