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Archive > Volume 25 Issue 10 > 2018,25(10):1021-1025. DOI:10.3872/j.issn.1007-385X.2018.10.008 Prev Next
Basic Research
Effects of RFX1 over-expression on proliferation and invasion of glioma cells
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Abstract:
Objective: To validate the effect and the possible mechanism of human regulatory factor X1 (RFX1) over-expression on the proliferation and invasion of glioma F98 cells. Methods: RFX1-overexpressed F98 cells (F98-RFX1 group) were constructed by lentivirus transfection, a control group (F98-Vector group) and normal group (F98 group) were established. The effect of RFX1 over-expression on F98 cell proliferation was observed with counting method, cell apoptosis was determined by AnnexinV-PI staining, and the cell invasion was observed with Transwell method. Results: F98 cell line over-expressing RFX1 was successfully established. The proliferation capacity of F98-RFX1 group was significantly lower than that of F98 group (48 h: [12.08±2.17]×104/ml vs [23.67±4.51]×104/ml, P<0.05] and F98-Vector group (96 h: [8.17±0.31]×104 /ml vs [18.58±1.18]×104 /ml, P<0.05); The apoptosis level of cells in F98-RFX1 group was significantly increased ([21.89±2.33]% vs [3.38±1.39]%, [10.42±1.83]%, P<0.05]; The invasiveness of cells in F98-RFX1 group was significantly reduced ([33.3±7.99] vs [56.5±13.9], [60.6±11.8], P<0.01). Conclusion: RFX1 can regulate the expression of genes related with proliferation and invasion, thereby inhibiting the proliferation and invasion ability of glioma cells and promote cell apoptosis.
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Project Supported:
Project supported by the Science and Technology Development Foundation of Fenyang College of Shanxi Medical University (No. 2016B05)
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