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Archive > Volume 27 Issue 10 > 2020,27(10):1100-1105. DOI:10.3872/j.issn.1007-385X.2020.10.005 Prev Next
Basic Research
miR-423-5p enhances chemoresistance of glioblastomas cells to temozolomide by regulating PDCD5
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Abstract:
Objective: To investigate the miR-423-5p expression in brain glioma tissues and cell lines, and its promotive effect on temozolomide (TMZ) chemoresistance by targeting PDCD5 (programmed cell death protein 5). Methods: Tumor tissues and matched peritumoral tissues were collected from 20 brain glioma patients who were surgically treated in the Department of Neurosurgery, Affiliated Hospital of Beihua University between January 2017 and December 2018. Glioblastoma cell lines (U251, U87, SHG-44) and human normal glial cell line HMC-3 were also used in the study. The relative expression of miR-423-5p and PDCD5 in brain glioma and peritumoral tissues andcelllineswasdetected by qPCR.ThesynthesizedmiR-423-5p mimics and miR-NC were respectively transfected into U251 and U87 cells; meanwhile,TMZatdifferentconcentrations (50, 100, 150 and 200 μmol/L) werealsousedtotreat thecells. Then, the chemoresistance of cells to TMZ were determined. MTT assayandcolonyformationassaywereusedtoexaminethe proliferationofU251andU87cells,andWesternblotting was used to detect the expression of c-caspase 3, Bcl-2 and PDCD5 proteins in U251 and U87 cells. The targeting relationship between PDCD5 and miR-423-5p was validated through Dual luciferasereportergene assay. Results: miR-423-5p was highly expressed in glioma tissues and glioma cell lines (all P<0.01). As compared with the miR-NC group, the proliferation and TMZ-chemoresistance of U251 and U87 cells in miR-423-5p mimics group significantly increased (all P<0.01). Dual luciferase reporter gene assay validated that miR-423-5p could bind with PDCD5 3' UTR to suppress the expression of PDCD5. Conclusion: High expression of miR-423-5p enhances the chemoresistance of glioma cells to TMZ, and miR-423-5p may serve as a potential therapeutic target in the treatment of brain glioma.
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Project Supported:
Project supported by the“13th Five-Year Plan”Science and Technology Project of the Education Department of Jilin Province (No. JJKH20180332KJ)
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