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Archive > Volume 27 Issue 11 > 2020,27(11):1206-1212. DOI:10.3872/j.issn.1007-385X.2020.11.002 Prev Next
Basic Research
miR-202 inhibits growth, migration and invasion of oral squamous cell carcinoma cells by down-regulating NFATc3
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Abstract:
Objective: To investigate the effect of miR-202-5p on the viability, colony formation, migration and invasion of oral squamous cell carcinoma (OSCC) cells, and to elucidate its possible mechanism. Methods: The mRNA expression levels of miR-202-5p and nuclear factor of activated T-cells isoform c3 (NFATc3) in OSCC cell lines(Tca8113 and SCC-4) were detected by qPCR. miR-202-5p mimics or/and NFATc3 overexpression plasmids were transfected into Tca8113 cells and SCC-4 cells. Cell proliferation was detected by MTT assay and colony formation assay; cell migration and invasion were detected by Wound-healing assay and Transwell assay; the expression level of NFATc3 protein was detected by Western blotting. The direct regulation of miR-202-5p on the candidate target gene NFATc3 was verified by Dual luciferase reporter gene assay. Results: Compared with the normal oral keratinocytes HOK, the expression level of miR-202-5p was significantly decreased in OSCC cell lines (P<0.01), and the mRNA and protein expressions of NFATc3 were significantly increased (P<0.01). Over-expression of miR-202-5p significantly inhibited cell viability, colony formation,migration, invasion, and the expression level of NFATc3 in Tca8113 cells and SCC-4 cells (all P<0.01). NFATc3 was proved to be a target gene of miR-202-5p. Over-expression of NFATc3 could reverse the inhibitory effect of miR-202-5p on the growth, migration and invasion of OSCC cells. Conclusion: miR-202-5p exerts its tumor suppressor function by down-regulating NFATc3 directly, leading to the inhibition of growth, migration and invasion of OSCC cells.
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Project Supported:
Project supported by the Medical Research Project of Health Commission of Chongqing (No. 2016MSXM046)
Clc Number:
