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Archive > Volume 27 Issue 11 > 2020,27(11):1220-1228. DOI:10.3872/j.issn.1007-385X.2020.11.004 Prev Next
Basic Research
circRNA_001569 affects the malignant biological behavior of breast cancer cells through the miR-145/HBXIP axis
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Abstract:
Objective: To investigate the role of circRNA_001569 in the proliferation, invasion and migration of breast cancer cells via miR-145/HBXIP axis. Methods: Collected 30 cases of cancerous tissue and 30 adjacent tissues of breast cancer patients treated in the Hengshui People's Hospital from January 2016 to January 2019. qPCR detects the expression of circRNA_001569 in breast cancer tissues, adjacent tissues and cell lines. Bioinformatics tools were used to predict the target genes of miR-145. RNA immunoprecipitation (RIP) and Dual luciferase reporter gene assay were used to detect the interaction between genes or proteins. si-circRNA_001569,miR-145 mimics or miR-145 inhibitor were transfected into breast cancer MDA-MB-231 and MCF-7 cells to establish gene overexpression or silence cell models. qPCR and Western blotting were used to detect the expressions of related genes and proteins.CCK-8 method and Transwell test were used to detect the proliferation, invasion and migration of transfected cells. Results: In breast cancer tissues and breast cancer cells, the expressions of circRNA_001569 and HBXIP were up-regulated, while the expression of miR-145 was down-regulated; RIP analysis and Dual luciferase analysis revealed the targeting relationship among miR-145,circRNA_001569 and HBXIP. Over-expression of circRNA_001569 or HBXIP promoted the proliferation, invasion and migration of MDA-MB-231 and MCF-7 cells (all P<0.01), while miR-145 overexpression had the opposite effect (all P<0.01). Conclusion:circRNA_001569 may promote the proliferation, invasion and migration of breast cancer cells by down-regulating the expression of miR-145 and up-regulating the expression of HBXIP.
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Project Supported:
Project supported by the Key Research and Development Project of Hebei Province (No.19277799D)
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