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Archive > Volume 27 Issue 11 > 2020,27(11):1264-1271. DOI:10.3872/j.issn.1007-385X.2020.11.009 Prev Next
Clinical Research
Research of tissue metabolomics in papillary thyroid carcinoma based on HPLC/Q-TOF-MS
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Abstract:
Objective: To explore the metabolic differences between papillary thyroid carcinoma (PTC) tissues and adjacent tissues using tissue metabolomics methods, and to search for potential biomarkers of PTC as well as to explore the pathogenesis and treatment strategies for PTC. Methods: Collected cancer and para-cancerous tissue specim ens from 40 patients with PTC who had undergone breast surgery at D epartm ent of Breast and Thyroid Surgery of H unan Provincial People's H ospital from O ctober 2018 to February 2020. Using the HPLC-MS technology platform, multi-dimensional statistical analysis of differential metabolites in PTC tissues and adjacent tissues was performed to find abnormal metabolic pathways related to PTC. Results: PCA, PLS-DA, OPLS-DA analyses showed that the metabolic profiles of cancer tissues and adjacent tissues were significantly different. OPLS-Loading plot analysis screened 76 potential differential metabolites (VIP>1, FC>2, and P<0.05). Among them, 33 metabolites such as Leucyl-phenylalanine,2-Oxomelatonin and Vanillactic acid were up-regulated in PTC tissues; 43 metabolites such as 3-glucoside, glycerophospholipid,phosphatidylcholine and lactose were down-regulated in PTC tissues. 13 abnormal metabolic pathways related to differential metabolites that may be involved in the pathophysiological process of PTC metabolism were found, such as: cysteine and methionine metabolism, glycerophospholipid metabolism, pyrimidine metabolism, galactose metabolism and alanine, aspartic acid and glutamic acid metabolism, citric acid cycle and so on. There were five types of differential metabolites with an area under the ROC curve greater than 0.9, namely pimelic acid, solerol, suberic acid, Alpha-Lactose and L-Serine. Conclusion: The galactose metabolism and amino acid metabolism in PTC tissues have changed, and Warburg effect exists in cells of PTC tissues. Pimelic acid, solerol, suberic acid,Alpha-Lactose and L-Serine can be used to distinguish PTC from normal people.
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Project Supported:
Project supported by the Clinical Medical Technology Innovation and Guidance of Hunan Province (No. 2018SK50720), the Natural Science Foundation of Hunan Province (No. 2017JJ3171), the Key Project of Health Commission of Hunan Province (A2017003), and the Science and Technology Project of Changsha City (No. kq1701045)
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