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Purple sweet potato anthocyanin regulates the proliferation, migration and invasion of breast cancer MDA-MB-231 cells through circ_0003998/miR-145 axis
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Abstract:
Objective: To investigate whether purple sweet potato anthocyanin (PSPA) regulates the proliferation, migration and invasion of breast cancer MDA-MB-231 cells through the circ_0003998/miRNA-145 axis. Methods: Breast cancer MDA-MB-231 cells were divided into control group, PSPA groups (200, 400, 800 μg/ml PSPA), pcDNA group, pcDNA-circ_0003998 group, si-NC group, si-circ_0003998 group, si-circ_0003998+anti-miR-145 group, PSPA+pcDNA group, PSPA+pcDNA-circ_0003998 group and PSPA+anti-miR-145 group. The expressions of circ_0003998 and miR-145 in MDA-MB-231 cells were detected by qPCR method. The cell proliferation, migration and invasion abilities were measured by CCK-8 method and Transwell chamber method, respectively. WB was used to detect the protein expressions of Ki-67, MMP-2 and MMP-9 in cells. Dual luciferase reporter gene assay was used to analyze the targeting relationship between circ_0003998 and miR-145. Results: Compared with the control group, the proliferation inhibition rate and miR-145 expression level in MDA-MB-231 cells of various PSPA groups increased significantly, while the expression of circ_0003998 and protein expression levels of Ki-67, MMP-2, MMP-9, as well as the number of migrated and invaded cells were significantly reduced (all P<0.01), all of which were in a concentration-dependent manner. circ_0003998 could target and negatively regulate the expression of miR-145. After inhibiting circ_0003998, the proliferation inhibition rate and the expression level of miR-145 in MDA-MB-231 cells were significantly increased, while the protein expression levels of Ki-67, MMP-2 and MMP-9, and the number of migrated and invaded cells were significantly reduced (all P<0.01). After co-transfection of si-circ_0003998 and anti-miR-145, the suppression effect of circ_0003998 inhibition on the proliferation, migration and invasion of MDA-MB-231 cells were reversed. circ_0003998 overexpression or miR-145 inhibition could reverse the inhibitory effects of PSPA on the proliferation, migration and invasion of MDA-MB-231 cells. Conclusion: PSPA inhibites the proliferation, migration and invasion of breast cancer MDA-MB-231 cells through the circ_0003998/miR-145 axis.
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Project Supported:
Project supported by the Scientific Research Project of Health and Family Planning System in Qinghai Province (No.2016-WJZD-10)
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