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Archive > Volume 28 Issue 8 > 2021,28(8):796-802. DOI:10.3872/j.issn.1007-385X.2021.08.005 Prev Next
Basic Research
Regorafenib regulates the proliferation and apoptosis of human hepatocellular carcinoma SMMC-7721 cells through miR-122
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Abstract:
Objective: To explore the effects of regorafenib (Rego) on proliferation and apoptosis of human liver cancer SMMC-7721 cells and the possible mechanism. Methods: SMMC-7721 cells were divided into control group and Rego group (10 μmol/L Rego).After the treatment for 48 h, Flow cytometry was used to detect cell apoptosis rate, and qPCR was used to determine the level of miR-122 in two groups of cells. hsa-miR-122-5p mimics were transfected into SMMC-7721 cells by lipofection to construct the miR-122 overexpressing cell line (overExp-miR-122). Then, the cells were divided into control group, Rego group, overExp-NC group,overExp-NC+Rego group, overExp-miR-122 group and overExp-miR-122+Rego group. MTT and Flow cytometry were used to detect cell viability and apoptosis rate, respectively. The protein expression levels of Bcl2, cleaved caspase-3, RAS, RAF1 and p-ERK1 in cells were detected by WB assay. Results: Compared with control group, the cell apoptosis rate was significantly increased after Rego treatment (P<0.05), and miR-122 expression level was significantly increased in Rego group (P<0.01). Compared with overExp-NC group, the proliferation inhibition rate and apoptosis rate in the cells of overExp-miR-122 group were significantly increased (P<0.01),the expression level of cleaved caspase-3 was significantly upregulated, while the protein expressions of Bcl2, RAS, RAF and p-ERK were significantly decreased (P<0.05 or P<0.01). Compared with overExp-miR-122 group, the changes in above detected indicators of overExp-miR-122+Rego group were more obvious (P<0.01). Conclusion: Regorafenib can inhibit the proliferation and promote apoptosis of SMMC-7721 cells, which may be achieved by regulating the expression of miR-122, thereby regulating the expression of apoptosis related factors and inhibiting RAS/RAF/ERK signaling pathway.
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Project Supported:
Project supported by the Local Science and Technology Development Special Project Guided by the Central Government from Hubei Province (No. 2019ZYYD067)
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