Mobile website
Basic Research
Up-regulation of lncRNA HOTTIP promotes the malignant biological behaviors of lung cancer SPC-A-1 cells through miR-637/KLK4 axis
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To investigate the effect and mechanism of lncRNA HOTTIP on proliferation, apoptosis and EMT of lung cancer cells. Methods: The expressions of lncRNA HOTTIP, miR-637 and KLK4 in SPC-A-1, BEAS-2B cells were detected by qPCR.After siRNA interference with the expression of lncRNA HOTTIP, the proliferation, invasion, apoptosis and EMT of SPC-A-1 cells were detected by CCK-8, Transwell, flow cytometry, and WB, respectively. The targeting relationship between lncRNA HOTTIP and miR-637 was analyzed by miRanda software and dual-luciferase reporter gene assay. RNA pull-down assay was used to detect the adsorption of lncRNA HOTTIP and miR-637, and to detect the effects of lncRNA HOTTIP regulating miR-637 on proliferation,invasion, apoptosis, and EMT of SPC-A-1 cells. The correlation between miR-637 and KLK4 was analyzed by TargetScan software,and the interaction between miR-637 and KLK4 was detected by dual-luciferase reporter gene assay. After siRNA interference with the expression of KLK4, the proliferation, invasion, apoptosis, and EMT of SPC-A-1 cells were detected. After down regulation of lncRNA HOTTIP and miR-637 expression, the levels of KLK4 mRNA and protein expression were detected by qPCR and WB. Results:Compared with BEAS-2B cells, the expression of lncRNA HOTTIP in SPC-A-1 cells was significantly up-regulated (P<0.01), the expression of miR-637 was down-regulated (P<0.01), the KLK4 expression was up-regulated (P<0.01). Down-regulation of lncRNA HOTTIP could significantly reduce the proliferation, invasion, and EMT capacity of SPC-A-1 cells, and increase the apoptosis rate (P<0.01). lncRNA HOTTIP had a targeting relationship with miR-637. Down-regulation of miR-637 expression could significantly promote the proliferation, invasion and EMT capacity of SPC-A-1 cells, and inhibit the apoptosis rate (P<0.01). miR-637 specifically bound to KLK4 3'UTR. Down-regulation of KLK4 could significantly inhibit the proliferation, invasion, and EMT capacity of SPC-A-1 cells, and increase the apoptosis rate (P<0.01). Down-regulation of lncRNA HOTTIP could significantly decrease KLK4 expression,while down-regulation of miR-637 could promote KLK4 expression (P<0.05). Conclusion: Up-regulation of lncRNA HOTTIP promotes proliferation, invasion, and EMT of lung cancer SPC-A-1 cells through miR-637/KLK4 axis, and inhibits the apoptosis of cancer cells.
Keywords:
Project Supported:
Project supported by the Self-funded Scien‐ tific Research Project of Guangxi Health Commission（No. Z20190032），and the Hospital Project of Liuzhou People's Hospital（No.lryjj201908）
Clc Number:
