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Expression of miR-627-3p in ESCC tissues and its effect on malignant biological behaviors of cancer cells
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Abstract:
Objective: To investigate the expression of miR-627-3p in esophageal squamous cell carcinoma (ESCC) tissues and its effect on biological behaviours of ESCC cells. Methods: From January 2015 to October 2015, 86 ESCC tissue specimens and 20 cooresponding adjacent normal tissue specimens from Thoracic Surgery Department of the Fourth Hospital of Hebei Medical University were collected. qPCR was used to detect the expression level of miR-627-3p in 86 cases of ESCC tissues and 20 cases of adjacent normal tissues. The relationship between miR-627-3p expression and clinicopathological parameters as well as prognosis was analyzed. The Kaplan-Meier plotter online database was used to further analyze the relationship between hsa-miR-627 expression and the prognosis of ESCC patients. qPCR was used to detect the expression of miR-627-3p in the four ESCC cells. miR-627-3p mimic was transfected into low-expressed cells, and miR-627-3p inhibitor was transfected into high-expressed cells. The cell proliferation was detected by CCK-8 assay, and the cell migration and invasion were detected by Transwell assay, respectively. KEGG analysis was used to explore the possible signal pathways mediated by miR-627-3p, and qPCR was used to verify the effect of miR-627-3p on the expression of key genes in the siganl pathway. Results: The expression of miR-627-3p in ESCC tissues was significantly lower than that in adjacent normal tissues. The expression of miR-627-3p was negatively correlated with lymph node metastasis and clinical stage of ESCC patients (all P<0.05). The five-year survival rate of ESCC patients with high miR-627-3p expression was significantly higher than that of the patients with low miR-627-3p expression (P<0.05). The expression of miR-627-3p was lowest in KYSE170 cells, and highest in KYSE30 cells. After transfection of miR-627-3p mimic into KYSE170 cells, the cell proliferation ability was not changed (P>0.05), but cell migration (P<0.05) and invasion (P<0.05) abilities were significantly decreased. After transfection of miR-627-3p inhibitor into KYSE30 cells, the cell proliferation ability was not changed (P>0.05), but cell migration (P<0.05) and invasion (P<0.05)abilities were significantly increased. KEGG analysis showed that miR-627-3p mediated multiple tumor-related signal pathways.Conclusion: The expression of miR-627-3p in ESCC tissues is significantly lower than in adjacent normal tissues, and its low expression is related to the poor prognosis of ESCC patients. miR-627-3p inhibits the migration and invasion of ESCC cells, which might play a biological function by interfering with multiple tumor-related signal pathways.
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Project Supported:
Project supported by the Medical Science Research Project of Hebei Province（No.20210074）
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