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linc00941 regulates the proliferation, invasion and glycolysis of esophageal squamous cell carcinoma via miR-203/CCL2 axis
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Abstract:
Objective: To explore the mechanism of linc00941 adsorbing miR-203 as ceRNA and up-regulating the expression of CC chemokine ligand 2 (CCL2) in esophageal squamous cell carcinoma (ESCC). Methods: The cancer tissues and adjacent tissues of 58 ESCC patients in the First Affiliated Hospital of Nanyang Medical College were selected, including 33 male patients aged (49.3±18.6) years and 25 female patients aged (44.6±20.7) years. The differential expression of linc00941, miR-203 and CCL2 in ESCC tissue, four human ESCC cell lines (EC9706, KYSE30, ECA109 and TE1) and human normal esophageal epithelial cell line HET-1A were detected by qRT-PCR. linc00941-wt, linc00941-mut, CCL2-wt and CCL2-mut plasmids were constructed and co transfected into 293T cells with miR-203 NC or miR-203 mimic, respectively. Dual luciferase reporter gene assay was implemented to verify the interaction between linc00941, miR-203 and CCL2. In addition, CCK-8 and Transwell experiments were used to detect the proliferation and invasion of cells. The lactic acid (LA) content was measured to evaluate the glycolysis ability of the cells. The cell apoptosis was detected by flow cytometry. Glycolysis inhibitor 2-DG and linc00941 were used to intervene ESCC cells to further observe the regulatory effect of linc00941 on ESCC cells. Results: The expressions of linc00941 and CCL2 were up-regulated while the expression of miR-203 was down-regulated in ESCC tissues and cell lines (all P<0.05). The interactions of linc00941 with miR-203 and miR-203 with CCL2 were both confirmed in ECA109 cells. Knockdown of linc00941 could inhibit the proliferation, invasion, glycolysis of ECA109 cells and induce their apoptosis, which was partly reversed by miR-203 inhibitor (all P<0.05). At the same time, CCL2 overexpression could partly reverse the effects of knockdown of linc00941 on the proliferation, invasion, glycolysis and apoptosis of ECA109 cells (all P<0.05). Conclusion: linc00941 can increase the expression of CCL2 via absorbing miR-203, subsequently promote the proliferation,invasion, glycolysis of ESCC cells and induce their apoptosis. The effects of linc00941 on the proliferation, invasion and apoptosis of ESCC cells may be realized by regulating glycolysis.
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Project Supported:
Project supported by the Key Project of Health Commission of Guangxi Zhuang Autonomous Region（No. 2020030）
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