Mobile website
Clinical Research
Down-regulation of miR-183 targeted tumor suppressor CPEB1 enhances radiosensitivity of glioma cells
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To investigate the effect of miR-183 on radiosensitivity of glioma cells.Methods: From October 2020 to June 2021, tissue samples of 40 cases of brain glioma from the First Hospital of Qinhuangdao were collected. T98G cells were irradiated with gradient dose X-ray (0, 2, 4, 6 Gy). The expression of miR-183 and CPEB1 in glioma tissues, T98G cells and X-ray irradiated T98G cells were detected by qPCR. After transfection of T98G cells with miR-183 inhibitor, the expression of miR-183 was down-regulated. The proliferation ability, apoptosis rate, BAX and Bcl2 protein expression of T98G cells were detected by CCK-8 assay, flow cytometry and WB, respectively, after 6 Gy X-ray vertical irradiation. The targeting relationship between miR-183 and CPEB1 was detected by Targetscan software prediction and dual luciferase reporter gene assay. After down regulating the expression of CPEB1, after 6 Gy X-ray irradiation, the proliferation ability, apoptosis rate, BAX and Bcl2 protein expression of T98G cells were detected by CCK-8 assay, flow cytometry and WB, respectively. T98G cells were transfected with pcDNA-CPEB1 or CPEB1 siRNA plasmid, and after CPEB1 was down-regulated or overexpressed, respectively, the effect of miR-183 on radiosensitivity of T98G cells through CPEB1 was detected.Results: The expression of miR-183 in glioma tissues and cells increased significantly, and the expression of CPEB1 mRNA decreased significantly. The expression of miR-183 in T98G cells decreased with the increase of X-ray radiation dose (P<0.05), and the expression of CPEB1 increased with the increase of X-ray radiation dose (P<0.05). After 6 Gy X-ray irradiation of T98G cells, down-regulation of miR-183 could reduce cell proliferation and increase apoptosis rate, while overexpression of miR-183 had the opposite effect (P<0.05).MiR-183 targeted mRNA CPEB1 and negatively regulated CPEB1 expression. After down-regulation of CPEB1, 6 Gy X-ray irradiation could significantly improve the proliferation of T98G cells (P<0.05) and reduce the apoptosis rate (P<0.05). MiR-183 could reverse the promoting effect of CPEB1 overexpression on radiosensitivity of T98G cells (P<0.05). Conclusion: Down-regulation of miR-183 expression can negatively regulate CPEB1, which might enhance the radiosensitivity of glioma cells.
Keywords:
Project Supported:
Project supported by the Key Medical Science Research Program of Hebei Province（No. 20181196）
Clc Number:
