Mobile website
Basic Research
Expression of lncRNA TOB1-AS1 in epithelial ovarian cancer tissues and its clinical significance
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Objective: To investigate the expression and clinical significance of transducer of ERBB2.1 antisense RNA1 (TOB1-AS1)in epithelial ovarian cancer (EOC) tissues, and to preliminarily explore its effect on the proliferation, migration, and invasion of EOC cells in vitro. Methods: TOB1-AS1 expression in EOC tissues was analyzed using the TCGA database. The tumor tissues from 67 patients who underwent tumor resection and were pathologically confirmed as EOC in the Department of Gynecology, the Fourth Hospital of Hebei Medical University from July 2017 to January 2018 were collected for this study. In addition, 30 non-tumor ovarian tissues of patients with other gynecological diseases were collected as the control in the same period. qPCR was used to detect the expression of TOB1-AS1 in EOC tissues and non-tumor ovarian tissues. χ2 test was used to analyze the correlation between the expression of TOB1-AS1 and different clinicopathological factors of EOC patients. Kaplan-Meier method and Cox proportional hazard regression model were used to analyze the survival and potential influencing factors for prognosis in EOC patients. The effects of TOB1-AS1 knockdown on the proliferation, migration, and invasion of EOC SKOV3 and A2780 cells were detected by CCK-8 test,Wound-healing assay, and Transwell test, respectively. Results: TCGA database analysis and qPCR results showed the expression level of TOB1-AS1 in EOC tissues was significantly higher than that in non-tumor ovarian tissues (all P<0.01). The high expression of TOB1-AS1 was conspicuously correlated with advanced FIGO stage, poor tissue grade, lymph node metastasis, and peritoneal metastasis in patients with EOC (all P<0.05). Kaplan-Meier survival analysis showed that post-operative disease-free survival (DFS)and overall survival (OS) in the patients with high TOB1-AS1 expression were shorter than those with low TOB1-AS1 expression (all P<0.05). Cox proportional hazard regression model analysis showed that FIGO stage, lymph node metastasis, peritoneal metastasis, and TOB1-AS1 expression were independent prognostic factors in EOC patients (all P<0.05). Similarly, the expression level of TOB1-AS1 in SKOV3 and A2780 cells were significantly higher than that in normal epithelial ovarian IOSE80 cells (all P<0.01). Cell function experiments showed that TOB1-AS1 knockdown inhibited the proliferation, migration, and invasion of SKOV3 and A2780 cells (all P<0.05). Conclusion: TOB1-AS1 is highly expressed in EOC tissues, and it is significantly related to the poor prognosis of EOC patients.TOB1-AS1 may affect the malignant progression of EOC by promoting the cell proliferation, migration, and invasion of SKOV3 and A2780 cells.
Keywords:
Project Supported:
Project supported by the National Natural Science Foundation of China（No. 81871894）
Clc Number:
