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Expression of lncRNA DNM3OS in laryngeal squamous cell carcinoma tissues and cells and its clinical and biological significance
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Abstract:
Objective: To explore the expression and clinical significance of lncRNA DNM3OS (dynamin 3 opposite strand/antisense RNA) in laryngeal squamous cell carcinoma (LSCC) tissues and cell lines, and to investigate its effects on in vitro proliferation,migration and invasion of LSCC TU177 cells, as well as to discuss the relationship between DNM3OS and epithelial-mesenchymal transition (EMT). Methods: Sixty-eight pairs of cancer and corresponding para-cancerous tissues from LSCC patients that admitted for surgery from March 2014 to December 2018 were collected from the biological specimen bank of the Fourth hospital of Hebei Medical University. The level of DNM3OS expression in LSCC tissues and cell lines was detected by qPCR. siRNA was used to knockdown DNM3OS expression in TU177 cell. MTS, colony formation and Transwell chamber assays were performed to detect the effect of DNM3OS knockdown on proliferation, migration and invasion of TU177 cells. qPCR and WB methods were used to detect the mRNA and protein expression of EMT-related markers, such as E-cadherin, N-cadherin, vimentin, twist, and SNAI2 after DNM3OS knockdown. Results: The expression level of DNM3OS in LSCC tissues was markedly higher than that in para-cancerous tissues (P<0.01). Upregulated DNM3OS expression was related to TNM stage, lymph node metastasis, and survival of patients with LSCC (P<0.05 or P<0.01). Furthermore, DNM3OS was highly expressed in five LSCC cell lines (Hep-2, AMC-HN-8, TU177, TU212, and TU686) (P<0.05 or P<0.01). The expression of DNM3OS was significantly decreased in TU177 cells after transfection of si-DNM3OS (P<0.01). Compared with the control group, DNM3OS knockdown could suppress the in vitro proliferation, migration and invasion of TU177 cells (all P<0.01), upregulate the expression level of E-cadherin, while down-regulate the expression of N-cadherin,vimentin, twist and SNAI2 in TU177 cells (all P<0.01). Conclusion: DNM3OS overexpression is related to the malignant progression of LSCC, which may be a potential prognostic marker for LSCC patients. DNM3OS may promote the invasion and metastasis of LSCC cells by mediating EMT.
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Project Supported:
Project supported by the Key Project of Medical Science Research of Hebei Province (No. 20180588, No. 20201511)
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