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Archive > Volume 29 Issue 4 > 2022,29(4):327-331. DOI:10.3872/j.issn.1007-385X.2022.04.008 Prev Next
Clinical Research
Expression of hsa_circ_0087378 in esophageal squamous cell carcinoma tissues and its effect of prognosis of patients
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Abstract:
Objective: To investigate the expression of hsa_circ_0087378 in esophageal squamous cell carcinoma (ESCC) tissues, and its effect on the clinicopathological characteristics and prognosis of ESCC patients. Methods: The differentially expressed circRNAs between ESCC tissues and corresponding para-cancerous tissues were screened by GEO database, which were then validated in 10 pairs of ESCC tissues and corresponding para-cancerous tissues by qPCR method. 90 pairs of cancer tissues and corresponding para-cancerous tissues resected from ESCC patients who admitted to the Fourth Hospital of Hebei Medical University from October 2015 to April 2016 were collected to detect the expression of hsa_circ_0087378 by FISH method, and the relationship between the expression of hsa_circ_0087378 and the clinicopathological indicators and prognosis of ESCC patients were also analyzed. Results: GEO database and qPCR analysis results showed that hsa_circ_0087378 was significantly up-regulated in ESCC tissues. FISH results showed that hsa_circ_0087378 were highly expressed in 21 (23.3%) of 90 cases of para-cancerous tissues, but in 65 (72.2%) of 90 cases of ESCC tissues. The hsa_circ_0087378 over-expression rate in ESCC tissues was significantly higher than that in para-cancerous tissues (P<0.05). The high expression of hsa_circ_0087378 in ESCC tissues was related to the clinical stage and lymph node metastasis of ESCC patients (P<0.05). The 5-year overall survival rate of patients with high hsa_circ_0087378 expression was significantly lower than that in patients with low circ_0087378 expression (P<0.05). Conclusion: hsa_circ_0087378 is highly expressed in ESCC tissues and positively correlated with the clinical stage, lymph node metastasis, and poor prognosis of patients, suggesting that hsa_circ_0087378 can be used as a potential therapeutic target for ESCC.
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Clc Number:
R735.1; R730.2; R730.7
