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Archive > Volume 29 Issue 4 > 2022,29(4):332-337. DOI:10.3872/j.issn.1007-385X.2022.04.009 Prev Next
Clinical Research
Characteristics and clinical significance of the changes in PD-1/PD-L1 and related immune cells in peripheral blood and tumor tissues of patients with cervical squamous cell cancer
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Abstract:
Objective: To investigate the changes in PD-1/PD-L1 pathway and related immune cells in the occurrence and development of cervical squamous cell cancer (CSCC) and their clinical significance. Methods: Cervical tissue/cancerous tissue and peripheral blood samples were collected from CSCC patients who underwent surgery and healthy controls who had physical examination at the First Hospital of Fuzhou City from December 2018 to September 2020 and were divided into healthy control group, cervical intraepithelial neoplasia (CIN) grade Ⅱ group, CIN grade Ⅲ group, and CSCC group to represent the occurrence and development of CSCC (n=50 in each group). The expression levels of PD-1, PD-L1 and forkhead box transcription factor P3 (FOXP3) in the peripheral blood of each group were detected by ELISA, the numbers of PD-1 + CD4 + CD25 + CD127 -/low cells in the peripheral blood of each group were detected by FCM, and the distribution of tumor-invasive lymphocytes (TIL) in CSCC tissues was detected by multicolor fluorescent immunohistochemistry. Results: The expression of PD-1, PD-L1 and FOXP3 in peripheral blood tended to increase with the onset and progression of mock CSCC and to decrease after surgery. The proportion of CD4 + , CD4 + CD25 + CD127 -/low and PD-1 + CD4 + CD25 + CD127 -/low cells in lymphocytes increased in the anticoagulated whole blood of CSCC patients. Massive infiltration of CD4 + , CD8 + and FOXP3 + cells was observed in CSCC tissues, with CD4 + and FOXP3 + cells mainly distributed around tumor cells and CD8 + and PD-L1 + cells widely and diffusely distributed. Conclusion: PD-1, PD-L1, FOXP3 and adaptive regulatory T cells are important factors contributing to the development of CSCC, which can be used as potential immunotherapeutic targets and potential prognostic markers for CSCC.
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Clc Number:
R737.33；R73-3
