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Archive > Volume 30 Issue 11 > 2023,30(11):1009-1015. DOI:10.3872/j.issn.1007-385X.2023.11.011 Prev Next
Clinical Research
Expression of DEAD box RNA helicases 10 and Bystin-like proteins in colorectal cancer tissues and cells and their clinical significance
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Abstract:
Objective: To investigate the correlation between DEAD box RNA helicases 10 (DDX10) and Bystin-like（BYSL）in colorectal cancer (CRC) tissues and colon cancer DLD-1 cells and its clinical significance. Methods: A total of 78 pairs of CRC tissues and corresponding paracancerous tissues surgically resected in the Second Affiliated Hospital of Fujian Medical University from March 2017 to March 2018 were collected for this study. The expression levels of DDX10 and BYSL in the cancer and paracancerous tissues were detected by immunohistochemistry EnVision method.SiDDX10 and siNC were transfected into DLD-1 cells by transient transfection technology, respectively. The effect of DDX10 expression on BYSL in colorectal cancer cells was detected by PCR. CCK-8 and transwell assays were used to verify the effect of DDX10 and BYSL on proliferation, migration and invasion of colorectal cancer cells. Results: The immunohistochemistry EnVision results showed that the expression levels of DDX10 (73.08%) and BYSL (74.36%) were significantly higher in colorectal cancer tissues compared with paracancerous tissues. The results of chi-square test showed that the differences in DDX10 and BYSL expression were statistically significant in CRC patients with different tumor stages and different lymph node metastasis and recurrence status (all P<0.05). Pearson correlation analysis showed that DDX10 expression waspositively correlated with BYSL expression (r=0.636, P<0.001). Kaplan-Meier survival analysis showed that the high expression of DDX10 and BYSL was correlated with a worse prognosis of patients. Logistic regression analysis proved that DDX10 and BYSL were independent risk factors for CRC recurrence. The results of CCK-8 assay and Transwell assay showed that suppressing the expression of DDX10 could decrease the expression of BYSL and inhibit the proliferation, migration and invasion of colorectal cancer cells (all P<0.05). Conclusion: DDX10 and BYSL are highly expressed in CRC tissues, and they are positively associated with each other. Higher expression levels of DDX10 and BYSL are associated with poorer patient prognosis and were independent risk factors for CRC recurrence. Suppressing the expression of DDX10 can decrease the expression of BYSL, and inhibit the proliferation, migration and invasion of colorectal cancer cells.
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