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Archive > Volume 32 Issue 4 > 2025,32(4):378-385. DOI:10.3872/j.issn.1007-385X.2025.04.005 Prev Next
Basic Research
CircPUM1 regulates malignant biological behaviors of endometrial cancer Ishikawa cells through the miR-337-3p/NPM1 axis
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Abstract:
[Abstract] Objective: To investigate the effect of circular RNA (cireRNA) pumilio RNA binding family member 1 (PUM1) on the proliferation, migration, invasion and apoptosis of endometrial cancer (EC) Ishikawa cells by regulating the miR-337-3p/ nucleophosmin 1 (NPM1) axis. Methods: Ishikawa cells were selected and plasmid sh-circPUM1 and its negative control (sh-NC), anti-miR-337-3p and its negative control (anti-NC) were transfected into Ishikawa cells by RNA interference. The experiment cells were divided into the control group (non-transfected cells), sh-NC group, sh-circPUM1 group, sh-circPUM1 + anti-NC group and sh-circPUM1 + anti-miR-337-3p group. The qPCR method was applied to detect the expressions of circPUM1, miR-337-3p, and NPM1 mRNA in Ishikawa cells in each group. CCK-8 method, EdU staining method, Transwell assay, and flow cytometry were applied respectively to detect the effects of knocking down circPUM1 on the proliferation, migration, invasion and apoptosis of Ishikawa cells. Western blot was applied to detect the changes in the expressions of PCNA, NPM1, MMP-9, SNAIL, E-cadherin, BAX and C-caspase-3 proteins in Ishikawa cells. Dual luciferase reporter gene experiment was applied to verify the targeting relationship between circPUM1 and miR-337-3p, and between miR-337-3p and NPM1. Results: Cell proliferation ability, EdU positive cell rate, migration and invasion numbers, circPUM1, NPM1 mRNA and protein, the expressions of PCNA, NPM1, MMP-9 and SNAIL protein in Ishikawa cells in the sh-circPUM1 group were significantly lower than those in the sh-NC group and Control group (all P < 0.05); the apoptosis rate, the expressions of miR-337-3p, E-cadherin, BAX, and C-caspase-3 proteins were significantly higher than those in the sh-NC group and the Control group (all P < 0.05). Compared with those in the sh-circPUM1 group and the sh-circPUM1 + anti-NC group, the apoptosis rate, miR-337-3p, the expressions of E-cadherin, BAX, and C-caspase-3 proteins in the sh-circPUM1 + anti-miR 337-3p group were significantly lower (all P < 0.05); Cell proliferation ability, EdU positive cell rate, migration and invasion numbers, NPM1 mRNA and protein, the expressions of PCNA, NPM1, MMP-9, and SNAIL proteins were significantly higher (all P < 0.05). CircPUM1 might target and negatively regulate miR-337-3p, and miR-337-3p might target and negatively regulate NPM1. Conclusion: Knocking down circPUM1 can inhibit the malignant biological behavior of Ishikawa cells, which might be achieved by targeting the miR-337-3p/NPM1 axis.
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