Mobile website
Archive > Volume 32 Issue 6 > 2025,32(6):579-586. DOI:10.3872/j.issn.1007-385X.2025.06.003 Prev Next
Basic Research
Circular RNA hsa_circ_0081621 promotes malignant biological behavior of human laryngeal squamous cell carcinoma cells
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
[Abstract] Objective: To investigate the effects of circular RNA (circRNA) hsa_circ_0081621 on the malignant biological behaviors of human laryngeal squamous cell carcinoma AMC-HN-8 and TU177 cells. Methods: AMC-HN-8 and TU177 cells were routinely cultured. si-NC, si-hsa_circ_0081621, empty vector (vector), and hsa_circ_0081621 overexpression vector (hsa_circ_0081621-OE) were transfected into AMC-HN-8 and TU177 cells, namely si-NC, si-hsa_circ_0081621, vector, and hsa_circ_0081621-OE groups, respectively. The effects of knockdown or overexpression of hsa_circ_0081621 on the proliferation, migration, and invasion of AMC-HN-8 and TU177 cells were detected by CCK-8 assay, colony formation assay, scratch wound healing assay, and Transwell chamber assay. Results: Successful knockdown or overexpression of hsa_circ_0081621 was achieved in AMC-HN-8 and TU177 cells. hsa_circ_0081621 knockdown significantly inhibited while hsa_circ_0081621 overexpression significantly promoted the proliferation, migration, and invasion of AMC-HN-8 and TU177 cells (P < 0.01 or P < 0.001 or P < 0.0001). Conclusion: hsa_circ_0081621 promotes the malignant biological behaviors of human laryngeal squamous cell carcinoma AMC-HN-8 and TU177 cells.
Keywords:
Project Supported:
Clc Number:
