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Archive > Volume 32 Issue 6 > 2025,32(6):587-593. DOI:10.3872/j.issn.1007-385X.2025.06.004 Prev Next
Basic Research
Effects of CREB on migration, invasion, and cell cycle of prostate cancer PC3 cells
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Abstract:
[Abstract] Objective: To investigate the effects of cyclic adenosine monophosphate response element binding protein (CREB) on the malignant biological behaviors of prostate cancer PC3 cells. Methods: Prostate cancer PC3 cells were routinely cultured. The overexpression control plasmid (vector), CREB overexpression plasmid (CREB-oe), knockdown control sequence (si-NC), and si-CREB sequence were transfected into PC3 cells using transfection reagents, namely vector, CREB-oe, si-NC, and si-CREB groups. Scratch wound healing assay, Transwell chamber assay, and flow cytometry were performed to evaluate cell migration, invasion, and cell cycle distribution, respectively. PC3 cells with CREB knockout were constructed using CRISPR/Cas9 technology, and a xenograft tumor model was employed to evaluate the impact of CREB knockout on tumor growth in vivo. Results: CREB was successfully knocked down or overexpressed in PC3 cells (all P < 0.01). CREB overexpression significantly promoted, while its knockdown significantly inhibited the migration and invasion of PC3 cells (all P < 0.01). Overexpression of CREB promoted the transition of PC3 cells into the S phase, whereas knockdown of CREB induced G1 phase arrest (all P < 0.01). PC3 cells with CREB knockout were successfully constructed, and CREB knockout significantly inhibited the growth of PC3 cell-transplanted tumors (P < 0.01). Conclusion: Knockdown or knockout of CREB inhibits migration and invasion of PC3 cells and induces G1 phase arrest, thereby suppressing tumor growth.
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