Mobile website
Archive > Volume 32 Issue 6 > 2025,32(6):594-603. DOI:10.3872/j.issn.1007-385X.2025.06.005 Prev Next
Basic Research
The effect of nectin-4/vanin-1 regulatory axis on the development of esophageal squamous carcinoma and the preliminary investigation of the mechanism
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
[Abstract] Objective: To explore the expression of nectin-4 and vanin-1 in esophageal squamous cell carcinoma (ESCC) and its influence on the malignant biological behaviors of ESCC cells, as well as the underlying mechanisms. Methods: Transcriptome sequencing combined with GO and KEGG enrichment analysis was used to identify the downstream target gene (vanin-1) regulated by nectin-4. The mRNA expression of vanin-1 in ESCC tissues was studied using the Timer2.0 database, and the mRNA and protein expression of vanin-1 in normal esophageal epithelial HET-1 and ESCC cells was detected by qPCR and Western blot, identifying ESCC KYSE-410 and KYSE-510 cells with the most significant differential expression. The expression of vanin-1 in KYSE-410 and KYSE-510 cells was knocked down using siRNA. The effects of vanin-1 knockdown on cell proliferation, migration, and invasion were measured using CCK-8 assay, wound healing assay, and Transwell chamber assay. Furthermore, KEGG and GO enrichment analyses were conducted for vanin-1-related signaling pathways. Immunohistochemistry was performed to compare the expression of vanin-1 between ESCC tissues and adjacent non-tumor tissues. Results: Timer2.0 database analysis and qPCR results showed that vanin-1 was highly expressed in both ESCC tissues and cell lines (both P < 0.01). WB assay also confirmed high expression of vanin-1 protein in ESCC cells (P < 0.01). siRNA successfully knocked down vanin-1 expression in KYSE-410 and KYSE-510 cells. Knockdown of vanin-1 significantly inhibited the proliferation, migration, and invasion capabilities of KYSE-410 and KYSE-510 cells (P < 0.05 or P < 0.01 or P < 0.001 or P < 0.000 1). KEGG and GO enrichment analysis suggested that vanin-1 might function through pathways related to pantothenic acid and coenzyme A synthesis metabolism. Immunohistochemistry results indicated that vanin-1 was highly expressed in ESCC tissues (P < 0.000 1). Conclusion: Vanin-1 is highly expressed in ESCC tissues and promotes the proliferation, migration, and invasion of KYSE-410 and KYSE-510 cells through the nectin-4/vanin-1 axis. Targeting vanin-1 might offer a new therapeutic strategy for ESCC.
Keywords:
Project Supported:
Clc Number:
