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Archive > Volume 32 Issue 6 > 2025,32(6):611-619. DOI:10.3872/j.issn.1007-385X.2025.06.007 Prev Next
Basic Research
Bioinformatics-based identification of a disintegrin and metalloprotease 8 as a potential shared target for inflammatory bowel disease and colorectal cancer
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Abstract:
[Abstract] Objective: To screen potential comorbid genes shared between inflammatory bowel disease (IBD) and colorectal cancer (CRC), and to explore the relationship between the key gene a disintegrin and metalloprotease 8 (ADAM8) and the pathogenesis of IBD and CRC, as well as the underlying mechanisms. Methods: Transcriptomic data and corresponding clinical information for IBD and CRC were downloaded from the GEO database and TCGA database. Differential expression analysis, prognostic gene screening, and intersection analysis were performed to identify shared genes. Multiple datasets were used to analyze and validate the expression patterns of ADAM8 in IBD and CRC and its correlation with clinicopathological features. Survival analysis was conducted to evaluate the prognostic value of ADAM8 in CRC. Functional and pathway enrichment analyses were conducted to explore the potential mechanisms by which ADAM8 influences CRC progression. The correlation between ADAM8 and tumor microenvironment components was further assessed using tumor microenvironmental algorithms. The database data was validated by detecting the expression in Chinese CRC tissues using immunohistochemistry (IHC). Results: ADAM8 was identified as a potential shared comorbidity gene in IBD and CRC. ADAM8 was significantly upregulated in both IBD and CRC tissues (all P < 0.01), and its high expression was associated with disease progression (P < 0.01). CRC patients with high ADAM8 expression had shorter overall survival (OS) (P < 0.05 or P < 0.01). ADAM8 was also significantly highly expressed in Chinese CRC tissues (P < 0.01). Pathway analysis revealed that ADAM8 expression was closely linked to immune cell migration, cytokine production, and immune receptor interactions. Additionally, ADAM8 expression positively correlated with immune cell infiltration, including neutrophils and macrophages (P < 0.01 or P < 0.001). Conclusion: ADAM8 is highly expressed in IBD and CRC tissues and is closely associated with patient prognosis, disease progression, and immune cell infiltration. It holds promise as a common therapeutic target for both diseases.
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