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Archive > Volume 32 Issue 10 > 2025,32(10):1044-1052. DOI:10.3872/j.issn.1007-385X.2025.10.006 Prev Next
Basic Research
Effects of miR-7-5p on the proliferation, apoptosis, and immune escape of esophageal squamous cell carcinoma KYSE-150 cells by regulating FOXM1
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Abstract:
[Abstract] Objective: To investigate the effects of miR-7-5p on the proliferation, apoptosis, and immune escape of esophageal squamous cell carcinoma (ESCC) KYSE-150 cells by regulating forkhead box M1 (FOXM1). Methods: The targeted binding sites of miR-7-5p and FOXM1 were verified by the dual-luciferase reporter gene assay. The cancer tissues and adjacent paracancerous tissues as well as the basic clinical data of 56 ESCC patients hospitalized at Changzhou Geriatric Disease Hospital affiliated to Soochow University between January 2022 and October 2024 were collected. qRT-PCR was used to detect the expression levels of miR-7-5p and FOXM1 in ESCC tissues, and analyze the relationship between their expressions and clinicopathological characteristics. Normal cultured KYSE-150 cells were selected as the Control group Plasmids were transfected into KYSE-150 cells using Lipofectamine 3000 transfection reagent, and the cells were assigned into the Mimic-NC group, the miR-7-5p mimic group, the miR-7-5p mimic + OE-NC group, and the miR-7-5p mimic + OE-FOXM1 group. EdU staining and CCK-8 assay were used to detect the proliferation ability of KYSE-150 cells. Flow cytometry was performed to detect the apoptosis of KYSE-150 cells and CD8+ T cells. Western blot assay was performed to detect the expression levels of PD-L1, FOXM1, BAX, and PCNA proteins in KYSE-150 cells. A nude mouse transplanted tumor model of KYSE-150 cells was established to observe the effects of miR-7-5p overexpression on the growth of the transplanted tumors and the expressions of Ki-67 and FOXM1 in the tissues. Results: miR-7-5p could target and negatively regulate FOXM1 (P < 0.05). miR-7-5p expression was low and FOXM1 expression was high in ESCC tissues (both P < 0.05). The expressions of miR-7-5p and FOXM1 were significantly correlated with TNM stage and differentiation degree, respectively (all P < 0.05). The rates of EdUpositive cells, cell proliferation ability, CD8+ T cell apoptosis rate, as well as PD-L1, PCNA, and FOXM1 mRNA and protein in the miR-7-5p overexpression group were significantly reduced (all P < 0.05), while the apoptosis rate, miR-7-5p, and BAX increased significantly (all P < 0.05). Meanwhile, overexpression of FOXM1 could reverse the above effects (all P < 0.05). Overexpression of miR-7-5p decreased the mass and volume of transplanted tumors, and the expressions of Ki-67 and FOXM1 proteins in transplanted tumor tissues (all P < 0.05). Conclusion: Overexpression of miR-7-5p can significantly inhibit the proliferation and immune escape of KYSE-150 cells and promote cell apoptosis, which may be achieved by targeted negative regulation of FOXM1.
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