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Archive > Volume 32 Issue 10 > 2025,32(10):1060-1064. DOI:10.3872/j.issn.1007-385X.2025.10.008 Prev Next
Basic Research
Germacrone suppresses the proliferation, migration, and invasion of prostate cancer PC3 cells by activating the cGAS-STING pathway
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Abstract:
[Abstract] Objective: To investigate the effects of germacrone (GM) regulation of the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon gene (STING) signaling pathway on the proliferation, migration, invasion and apoptosis of prostate cancer (PCa) cells. Methods: PCa PC3 cells were randomly separated into the control group (normal culture), the L-GM, the M-GM, the H-GM groups (each treated with 120, 240, 480 μmol/L GM respectively), and the GM + RU.521 group (treated with 480 μmol/L GM + 1 μmol/L cGAS inhibitor RU.521). EdU staining, CCK-8 assay, scratch assay, Transwell assay, and flow cytometry were applied to detect the effects of GM on the proliferation, migration, invasion, and apoptosis of PC3 cells, respectively. WB assay was used to detect the effects of GM on the expressions of cGAS and STING proteins in PC3 cells. Results: Compared with those in the control group, the rate of EdU-positive cells, cell proliferation activity, scratch healing rate, and the number of invasive cells in the L-GM, M-GM, and H-GM groups were significantly reduced (all P < 0.05); the apoptosis rate increased (P < 0.05); the expressions of cGAS and STING proteins were significantly upregulated, and were concentration-dependent (all P < 0.05). Compared with those in the H-GM group, the rate of EdU positive cells, cell proliferation activity, scratch healing rate and the number of invasive cells in the GM + RU.521 group were significantly elevated (all P < 0.05); the cell apoptosis rate decreased (P < 0.05); and the expressions of cGAS and STING proteins were significantly downregulated (all P < 0.05). Conclusion: GM inhibits the proliferation, migration, invasion and promotes the apoptosis of PCa cells by activating the cGAS-STING signaling pathway.
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