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Archive > Volume 33 Issue 6 > 2026,33(6):670-677. DOI:10.3872/j.issn.1007-385X.2026.06.009 Prev Next
Basic Research
Effect of oenothein B on the malignant biological behaviors of ovarian cancer SKOV3 cells by regulating the PI3K/AKT/NF-κB signaling pathway
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Abstract:
[Abstract] Objective: To investigate the effect of oenothein B on the malignant biological behavior of ovarian cancer SKOV3 cells by regulating the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/nuclear factor-kappa B (NF-κB) pathway. Methods: The half-maximal inhibitory concentration (IC50) of oenothein B on SKOV3 cells was determined by the CCK-8 assay, and the subsequent experimental doses were determined based on the IC50 value and relevant literature. SKOV3 cells were divided into the control group, low-dose oenothein B group (6.25 μmol/L), medium-dose oenothein B group (12.5 μmol/L), high-dose oenothein B group (25 μmol/L), PI3K inhibitor (LY294002) group, and high-dose oenothein B combined with PI3K activator (740Y-P) group. The cell proliferation ability of each group was detected by CCK-8 assay and 5-ethynyl-2'-deoxyuridine (EdU) staining. Transwell invasion assay, wound healing assay and flow cytometry were used to detect cell invasion, migration ability and apoptosis in each group, respectively. RT- qPCR was applied to detect the mRNA expression levels of proliferation-related gene Cyclin D1, migration-related gene migration and invasion enhancer 1 (MIEN1), and apoptosis-related gene p53 in each group. Western blotting was used to determine the expression levels of PI3K/AKT/NF-κB pathway-related proteins including p-PI3K, p-AKT and NF-κB p65 in each group. Results: The IC50 value · · 670 [[PAGE_INDEX=2 FILE=37dae296-6188-4ec2-9b39-45b08e05f9a4.pdf]] 张颖, 等. 月见草素B调控PI3K/AKT/NF-κB通路对卵巢癌SKOV3细胞恶性生物学行为的影响of oenothein B against SKOV3 cells after 24 h of treatment was 28.70 μmol/L. Compared with the control group, the proliferation ability, number of invasive cells and wound healing rate of SKOV3 cells were significantly decreased in the low-, medium- and high- dose oenothein B groups as well as the LY294002 group (all P < 0.05); the mRNA expression levels of Cyclin D1 and MIEN1, as well as the protein expression levels of p-PI3K, p-AKT and NF-κB p65 were markedly downregulated (all P < 0.05), while the cell apoptosis rate and p53 mRNA expression were remarkably upregulated (all P < 0.05). Compared with the LY294002 group, the high-dose oenothein B exhibited more pronounced inhibitory effects on cell proliferation, invasion, and migration (all P < 0.05), as well as greater downregulation of Cyclin D1 and MIEN1 mRNA and p-PI3K, p-AKT, and NF- κB p65 protein expression, and more significant upregulation of apoptosis rate and p53 mRNA expression (all P < 0.05). Compared with the high-dose oenothein B group, the high-dose oenothein B + 740Y-P group showed increased cell proliferation, invasion, and migration (all P < 0.05), upregulated Cyclin D1 and MIEN1 mRNA and p-PI3K, p-AKT, and NF-κB p65 protein expression (all P < 0.05), and decreased apoptosis rate and p53 mRNA expression (all P < 0.05). Conclusion: Oenothein B may inhibit the PI3K/AKT/NF- κB signaling pathway, thereby suppressing the proliferation, invasion and migration of ovarian cancer SKOV3 cells and inducing cell apoptosis.
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